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EM Consulting
Information & C.V.
Elvin A. Woodruff III, Magnify Inc.
Electron Microscopy Specialist
Nashville, Tennessee 37235-1634
Cell phone (615) 428-7776
E-mail contact eawoodruff@live.com
CURRICULUM VITAE
Education/Degrees:
Tennessee State University 1991 Bachelor of Science (Animal Science)
Qualifications:
-Detection of nanoscale materials/structures in biological samples
-Technical guidance or independently conducting exceptionally difficult research
-Review, appraisal and interpretation of experimental results
-Prepare publications
-Prepare presentations
Excellent communication and interpersonal skills
-Software tools for automated image analysis
-Expert level knowledge of windows software and architecture
-Highly proficient in the use of Microsoft Office suite of programs
-High resolution TEM and STEM imaging
-Specialized ultrastructural procedures (immune-electron microscopy, serial sectioning, negative staining, particle counting, microwave processing)
-Perform periodic maintenance on instruments and minor repairs as needed
Maintain accurate department records, supply inventories and departmental purchase records
-Consultation with investigators regarding the special nature of their projects and determine wheather projects are suitable or adaptable to examination
Equipment:
-EDAX
-Phillips 300, 301,CM10, CM12, JEOL 6701F, Morgagni
-Microtome (paraffin sections)
-Cryo-techniques
-Leica high pressure freezer
-Balzers HPM 010 High Pressure Freezer
-Freeze substitution system
-Cryo-ultramicrotome
-Leica Ultracut UCT ultramicrotome
-Glass knife maker
-Critical Point Dryer
-Suptter Coater
-Confocal
Bibliography:
1. Zhang, Y., Matthies, Mancuso, J., H. Andrews, H., Woodruff, E. Friedman, D. and Broadie, K. (2004). The Drosophila Fragile X-related gene regulates axoneme differentiation during spermatogenesis. Developmental Biology Jun 15;270(2):290-307.
2. Rohrbough, J., Rushton, E., Palanker, L., Woodruff, E., Mathies, H., Acharya, U., Acharya, J.K. and Broadie, K. (2004) Ceramidase Regulates Synaptic Vesicle Exocytosis and Trafficking. The Journal of Neuroscience Sep 8;24(36):7789-803.
3. Pan, L., Zhang, Y. Q., Woodruff, E. and Broadie, K. (2004). The Drosophila fragile X gene negatively regulates neuronal elaboration and synaptic differentiation. Current Biology Oct 26;14(20): 1863-70.
4. Zhang, Y., Friedman, D.B., Wang, Z., Woodruff, E., Pan, L., O’Donnell, J. and Broadie, K. (2005). Protein expression profiling of the Drosophila Fragile X mutant brain reveals upregulation of monoamine synthesis. Cellular and Molecular Proteomics Mar;4(3):278-90. [Epub 2005 Jan 4.
5. Dong, X., Li, H., Derdowski, A., Ding, L., Burnett, A., Chen, X., Peters, T.R., Dermody, T.S., Woodruff, E., Wang, J.J. and Spearman, P. (2005). AP-3 Directs the Intracellular Trafficking of HIV-1 Gag and Plays a Key Role in Particle Assembly. Cell Mar 11;120(5): 663-674.
6. Huang, F.D., Woodruff, E., Mohrmann, R. and Broadie, K. (2006). Rolling blackout is required for synaptic vesicle exocytosis. Journal of Neuroscience Mar 1;26(9):2369-79.
7. Haas, K.F., Woodruff, E. and Broadie, K. (2007). Proteasome function is required to maintain muscle cellular architecture. Biology of the Cell Nov;99(11): 615-26.
8. Rohrbough, J., Rushton, E., Woodruff, E., Fergestad, T., Vigneswaran, K. and Broadie, K. (2007). Presynaptic establishment of the synaptic cleft extracellular matrix is required for post-synaptic differentiation. Genes & Development Oct 15;21(20):2607-28. [Epub 2007 Sep 27]
9. Kucenas S, Takada N, Park HC, Woodruff E, Broadie K, Appel B. (2008). CNS-derived glia ensheath peripheral nerves and mediate motor root development. Nature Neuroscience. Feb; 11(2):143-51[Epub 2008 Jan 6].
10. Long A.A.,Kim E. leung HT, Woodruff E. 3rd, An L, Doerge R.W., Pak W.L, Broadie K. (2008). Presynaptic calcium channel localization and calcium-dependent synaptic vesicle exocytosis regulated by the Fuseless protein. J. Neurosci. Apr. 2;28(14):3668-82.
11. Pan L., Woodruff E. III, Liang P, Broadie K. (2008). Mechanistic relationships between Drosophila fragile X mental retardation protein and metabotropic glutamate receptor A signaling. Mol Cell Neurosci. Apr;37(4):747-60. Epub 2008 Jan. 17.
12. Phillips S.E., Woodruff E. A. 3rd, liang P., Patten M., Broadie K. (2008). Neuronal loss of Drosophila NPC1a causes cholesterol aggregation and age-progressive neurodegeneration. J Neurosci. Jun. 25;28(26):6569-82.
13. Mohmann R., Matthies H.J., Woodruff, E. 3rd, Broadie K. (2008). Stoned B mediates sorting of integral synaptic vesicle proteins. Neuroscience. Jun. 2;153(4):1048-63. Epub 2008 mar.6.
14. Woodruff, E. A. III, Honegger, H. W., Broadie K. Two peptide transmitters co-packaged in a single neurosecretory vesicle. Peptides. 2008 Sep 20. [Epub ahead of print].
15. Vijayakrishnan, Niranjana, Woodruff, E. A. 3rd, Broadie, K. (2008). The Drosophila EFR3 Rolling Blackout is Required for Bulk Endocytosis in Non-Neuronal Cells and neuronal Synapses. J. of Cell Science (accepted).
Acknowledgements:
Melissa S. Maginnis, Bernardo A. Mainou, Aaron Derdowski, Elizabeth m. Johnson, Roy Zent, and Terence S. Dermody. NPXY motifs in the β1 integrin Cytoplasmic Tail Are Required for Functional reovirus Entry. J Virology, Apr. 2008, p.3181-3191
Arman S. kussainov, mark D. Does, John C. Gore and Daniel F. Gochberg. CRAZED signal dependence on correlation distance and sample orientation in rat sciatic nerve. August 5, 2008 (submitted)
Nikki Shariat, Robin C. C. Ryther, john A. Phillips III, Iain C. A. F. Robinson, and James G. Patton. Rescue of Pituitary Function in a Mouse Model of Isolated Growth Hormone Deficiency Type II by RNA Interference. Endocrinology, Feb.2008,149(2):580-586
Mildred T. Stahlman, Mary Phillips Gray, Mary W. Falconieri, Jeffrey A. Whitsett, and Timothy E. Weaver. Labortory Investigation, Mar. 2000, 80(3):395-403 Xuemin Chen, Michael T. Rock, Jason Hammonds, James Tartaglia, Ayumi Shintani, Jeff Currier, Bonnie Slike, James E. Crowe, Jr., Mary Marovich, and Paul Spearman. Pseudoviron Particle Production by Live Poxvirus Human Immunodeficiency Virus Vaccine Vector Enhances Humoral and Cellular Immune Responses. Journal of Virology, May 2005, p. 5537-5547
Cover:
The Journal of Neuroscience, June 25, 2008, Volume 28 Number 26
Calendar:
Biological Sciences, October 2007
webpage contact:
Nanosciences & BioTech