|
Studies on
Expression of Chlorophyll a/b Binding Protein Genes in Pachydandra
terminalis Using Real-time PCR
Suping
Zhou, Roger Sauve, Samuel Nahashon, Tingting Chen, Safdar Muhammad,
Sarabjit Bhatti, and Deborah Long
Institute of Agricultural and Environmental Research
Seminar Series
Tennessee State University, Nashville, TN
October 26, 2005
We have cloned two new chlorophyll a/b
binding protein genes from Pachysandra terminalis using cDNA
differential display, cDNA library screening and 5’-race PCR techniques.
The deduced amino acid sequence alignment showed that the two new genes
belong to LHCII type I and type II gene family. Real-time PCR was used
to study the response to cold treatments at gene transcription level.
Results showed that the 4C cold treatment can enhance transcription of
these genes.
Return
to Seminar Series
|
