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Seminar Series Abstract

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Entomology Program and Japanese Beetle Research

Catharine Mannion and Jason Oliver

Cooperative Agricultural Research Program Seminar Series
Tennessee State University, McMinnville, TN
March 10, 1999

Introduction.  The entomology program at the TSU Nursery Crop Research Station has been ongoing for approximately 3 years and is staffed with an Associate and Assistant Investigator. The researchers in this program investigate and research insect problems in the field-grown nurseries. There are currently 5 projects: Japanese beetle, imported fire ant, Asian ambrosia beetle, flatheaded apple tree borer, and the Midway beetle. The Japanese beetle project has been the primary project in the program. Japanese beetle is a regulated pest; numerous states and counties maintain exterior quarantine or certification requirements. Nursery stock shipped from infested areas to non-infested areas can potentially contain the immature stages of Japanese beetle, and therefore, are at risk of spreading this pest to non-infested areas.

Objectives.  The objectives of the Japanese beetle project are to develop management strategies to reduce the number of Japanese beetle grubs in the field-grown nurseries. There have been three major areas of focus: insecticides (use of new, low-impact compounds; application technology; formulation; rate; and timing), biological control [entomopathogenic nematodes (Heterorhabditis spp.), Bacillus thuringiensis, and Tiphia vernalis] and cultural control (clean cultivation, deterrents and barriers to oviposition).

Methodology.  Field tests have been conducted to evaluate treatments and application technologies for three years. Methodology varied among tests; however, all field tests were conducted and evaluated similarly. Treatments were applied at the appropriate times and rates around trees in commercial nurseries. At the time of evaluation, trees were dug with a mechanical digger and the root ball examined for scarab larvae. All scarab larvae were identified and counted. Cultural practices being evaluated, such as clean cultivation, were maintained during the period of Japanese beetle flight. Methods or treatments to deter oviposition were also examined. Laboratory studies were conducted on rearing a parasitoid of Japanese beetle (Tiphia vernalis) and the interaction of the parasitoid and commonly used pesticides. Laboratory studies were also conducted to examine the interaction between the entomopathogenic nematode, Heterorhabditis marelatus and an insecticide used against Japanese beetle larvae.

Results.  As a result of the insecticide work, a soil application of imidicloprid was approved as an acceptable treatment for regulatory control. Dipping root balls in chlorpyrifos also provided excellent results. Entomopathogenic nematodes and B. t. provided approximately 50% reduction in the grub population. Laboratory tests demonstrated no interaction between an insecticide used for grub control and an entomopathogenic nematode. Testing with Tiphia are ongoing.

Impact.  Japanese beetle is a serious problem for the nursery industry. The need for reliable, consistent treatments to reduce the risk of spreading this pest is tremendous. Currently, there are very few options available to growers; however, new options are now available as a result of this work. Additionally, data generated in this program will help establish a long-term, sustainable reduction in the Japanese beetle population. Information generated on cultural practices and biological control in conjunction with the use of low-impact, environmentally safe insecticides will help reduce the risk of spread.

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