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Institute of Agricultural & Environmental Research Tennessee State University |
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Seminar Series Abstract
Antimicrobial Resistance of Campylobacter, Salmonella in Chicken and Guinea Fowl A. KILONZO-NTHENGE, S. N. NAHASHON*, F. CHEN AND N. ADEFOPE Tennessee State University Institute of Agricultural & Environmental Research This study was conducted to compare the presence and antimicrobial susceptibility of Campylobacter, Salmonella spp, and other enteric bacteria between chickens and guinea fowls. Birds were reared on enclosed concrete floor housing covered with pine wood shavings litter material. Chicken (n=20) and guinea fowls (n=20) carcasses, drinking water (10 ml; n=20) and litter (10 g; n =20) were aseptically collected randomly from a poultry farm and analyzed within 1 h of collection. Individual pens served as experimental units and were replicated two times. Campylobacter ssp, Salmonella spp, and other enterobactericea were isolated and identified using standard selective media and biochemical tests and were tested for resistance to tetracycline, ampicillin, streptomycin, kanamycin, Nalidixic acid, gentamicin, erythromycin, ciprofloxacin, and cefoxitin, and colistin using the Kirby Bauer disc diffusion test. Campylobacter spp and Salmonella spp were isolated from 25 and 35%, and 15 and 20% of whole carcass rinses of chickens and guinea fowl, respectively. Although only Salmonella spp were recovered from drinking water, both Salmonella and campylobacter ssp were recovered in litter material. Campylobacter upsaliensis was recovered only in the guinea fowl while Klebsiella oxytoca and Enterobacter sakazakii were recovered only in chickens. While no antibiotic resistance was determined in Campylobacter upsaliensis, Campylobacter jejuni, Campylobacter lari, Salmonella and E. coli isolates from both chickens and guinea fowl were resistance to antibiotics such as ampicillin, kanamycin, erythromycin and nalidixic acid. (Key words: chickens, guinea fowl, antimicrobial resistance)
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