Biology 6560  Techniques in Light and Electron Microscopy

Summer I - 2008

 

Syllabus 

MICROSCOPY PRINCIPLES (Light and EM)

bullet

Classic Book: Principles of Microscopy: Being a Handbook to Microscope  (by Almroth Wright 1906)

bullet

Microscopy Presentation 1_Overview Concepts

bullet

Light Microscopy Concepts (Invisible/Visible)

bullet

Dark field

bullet

Fluorescence and Fluorescence Probes_Introduction to Confocal Microscopy and Image Analysis (Overview)

bullet

Important Concepts/Words

bullet

Microscopy Primers/Tutorials (Nikon)

bullet

Microscopy Primers/Tutorials (Olympus)

bullet

PCM 2000 Laser Scanning Confocal Microscope Guide-BASIC

bullet

PCM 2000 Laser Scanning Confocal Microscopy Guide_EXPANDED WITH APPLICATIONS

Electron Microscopy

bullet

Safety

bullet

EM History

bullet

Cell Ultra structure

bullet

Specimen Preparation (Buffers, Fixatives)

bullet

More on Factors Affecting Sample Preparation

bullet

More on Fixatives and Buffers

bullet

EM Fixatives (Recipes)

bullet

Dehydration, Embedding and Sectioning

bullet

Section Mounting, Support Films, Post-Staining

bullet

SEM Mounting, Drying, Coating

bullet

Shadow Coating and Replicas

bullet

Variable Pressure and Environmental SEM (ESEM)

bullet

Cryofixation for TEM and SEM 

bullet

Autoradiography

bullet

Immunocytochemistry

bullet

Labeling (Enzymes, Lectins, etc)

bullet

EM Artifacts

Microscopy Course Web Resources

Microscopy Site/Description

URL

Microscopy Primer http://www.microscopy-uk.org.uk/index.html?http://www.microscopy-uk.org.uk/primer/intro.htm 

BioEd Online-Microscopy

http://www.bioedonline.org/slides/slide01.cfm?tk=36&dpg=1

Google “brightfield microscopy”

http://www.google.com/search?hl=en&q=Bright+Field+Microscopy

Intro to Microscopy University

http://www.microscopy-uk.org.uk/index.html?http://www.microscopy-uk.org.uk/intro/illu/illu.html

Light Microscopy UK (Rice Univ)

http://www.geog.ucl.ac.uk/~jhope/lab/micro2.stm

Light/EM Microscopic Principles Techniques_ (Chapman-Microbiology)

UIUC

http://www.life.uiuc.edu/mcb/101/docs/microscopy_sp06.pdf

Microscopy_Invisible Visible

http://biology.fullerton.edu/biol302/302labf99/scope1.html

Molecular Expressions-Microscopy Primer

http://micro.magnet.fsu.edu/index.html

Microscopy in Cell Biology

http://www.biochemweb.org/microscopy.shtml

Polarized Light Microscopy Primer

http://www.olympusmicro.com/primer/techniques/polarized/polarizedintro.html

 

Fluorescence Microscopy Primer

http://www.olympusmicro.com/primer/techniques/fluorescence/fluorhome.html

Fluorochrome Data_Olympus

http://www.olympusfluoview.com/theory/fluorochromedata.html

Theory of Confocal Micr._Olympus Primer

http://www.olympusfluoview.com/theory/index.html or http://www.olympusfluoview.com/

Interactive Primers/Tutorials

Microscopy U (Nikon)

http://www.microscopyu.com/

 Interactive Microscopy Primers_Olympus

  http://www.olympusmicro.com/primer/virtual/virtual.html

  Microscopy Society of America

  http://www.microscopy.org/

Google “microscopy”

http://www.google.com/search?hl=en&q=Microscopy

  NIH Image (Mac OS only))

  http://rsb.info.nih.gov/nih-image/

  Image J (All OS)

  http://rsb.info.nih.gov/ij/

  Scion Image for Windows

  http://www.scioncorp.com/frames/fr_download_now.htm 

http://rclsgi.eng.ohio-state.edu/~mcferran/html/Scion_Image_PC_Manual.pdf (manual)

Nikon PCM 2000 Confocal

  http://www.pharm.stonybrook.edu/dept/instrumentation/NIKON%20PCM%202000/App%20Guide%203.6/3%20Applications%20Guide-Getting%20Started/Table%20of%20Contents.htm#TopOfPage

  Use of Fluorescence Proobes

  http://www.loci.wisc.edu/optical/probes.html

  Introduction to Fluorescence Techniques

http://probes.invitrogen.com/handbook/sections/0001.html

 

  Invitrogen/Molecular Probes-The Handbook — A Guide to Fluorescent Probes and Labeling Technologies

  http://probes.invitrogen.com/handbook/

  Fluorescence Resources-reagents, labels and probes

  http://www.fluorescence-resource.com/index.cfm?fig=Reagents

  Developmenal Studies Hybridoma Bank_Monoclonal Antibodies @ Univ. Iowa 

http://dshb.biology.uiowa.edu/commerce/catalog/antigen.jsp

 

Scanning Electron Microscope (SEM) Introduction http://www.chems.msu.edu/curr.stud/mse.sops/sem.intro.htm 

  Virtual Scanning Electron Microscopy

  http://micro.magnet.fsu.edu/primer/java/electronmicroscopy/magnify1/index.html